About Six Month Program

● The main objective of this program is to encourage the bright and well-motivated students of Kalinga University and other institutions to pursue innovative research and development of project work.

● The purpose of this internship is to enrich the learning experience by immersing students in an international level research activity along with the hands-on experience in certain aspects of Biotechnology (Scientific Research, Laboratory Work & Scientific Problem Solving) for academic credit.

Advantages

● It helps the students to develop the skills and competencies they require to become employable.

● It provides an opportunity for students to apply theoretical knowledge acquired in the classroom with practical application of knowledge required to perform a task.

● It gives them a feel of how their work environment will be when they get employed. Industrial training also gives exposure to students on the tools used in the Industry.

● Developments of in-hand biological science process skills enable students to better understand science and perform better during interviews. As practical skilled knowledge is the most important aspect of technology-driven science like Biotechnology or Bioinformatics.

● Internships provide students numerous perks: They gain experience, develop skills, make connections, strengthen their resumes, learn about a field, and assess their interests and abilities

Who Should Attend

BENEFICIAL FOR - Students of UG/PG Programs & Research Scholars of Biotechnology, Microbiology, Botany & Zoology

Program Details

Program Schedule

	Topic I-Agrobacterium Mediate Gene Transfer in Plants
Duration	Content
Module-1 January 2023	● Literature survey of Related topic ● Preparation of Culture Media to grow Agrobacterium ● Preparation of Culture of different Agrobacterium strains ● Growing appropriate plant and plantlets for transformation
Module-2 February 2023	Isolate genes of interest from the source organism: ● Isolation of Genomic DNA of Source organism ● Restriction Digestion and Southern blotting to isolate gene of interest
Module-3 March 2023	Insert the transgene into the Ti-plasmid: ● Construction of Recombinant DNA by integration of Gene of interest with Ti Plasmid ● Replacement of phytohormone biosynthesis gene with gene of interest
Module-4 April 2023	Introduce the T-DNA containing-plasmid into Agrobacterium: ● Agrobacterium transformation with recombinant Ti Plasmid ● Selecting transformed Agrobacterium using polymerase chain reaction (PCR) ● Simplified Arabidopsis transformation: Floral inoculating method ● Screening of transgenic plants by PCR
Module-5 May 2023	● Review of literature to collect data for report ● Dissertation report writing ● Corrections and editing
Module-6 June 2023	● Preparation of manuscript for publication ● Corrections, editing and submission to Journal

	Topic II-Gene Cloning in Bacteria
Duration	Content
Module-1 January 2023	Isolation of DNA (Gene of Inter¬est) Fragments to be cloned: ● Literature survey of Related articles ● Preparation of Culture Media for E. Coli Culture ● Isolation of Pure Culture of E. Coli ● Genomic DNA Isolation from host to get desired gene ● Restriction digestion and Southern Blotting to get desired gene
Module-2 February 2023	Insertion of Isolated DNA into a Suitable Vector to Form the Re-combinant DNA: ● Isolation and Selection of a suitable plasmid Vector ● Restriction Digestion of Cloning vector and Gene of Interest ● Construction of recombinant DNA plasmid
Module-3 March 2023	Introduction of the Recombinant DNA into a Suitable Organism known as Host: ● Preparation of Competent E. coli cells ● Transformation of Recombinant plasmid into host cells ● Selection of transformed and non-transformed cells
Module-4 April 2023	Selection of the Transformed Host Cells and Identification of the Clone Containing the Gene of Interest: ● Selection of transformed and non-transformed cells ● Identification of Recombinant and Non-recombinant cells ● Multiplication/Expression of the Introduced Gene in the Host ● Isolation of the Multiplied Gene Copies/Protein Expressed by the Intro¬duced Gene
Module-5 May 2023	● Review of literature to collect data for report ● Dissertation report writing ● Corrections and editing
Module-6 June 2023	● Preparation of manuscript for publication ● Corrections, editing and submission to Journal

	Topic III-A Study on Different Gene Delivery Approaches Used in Genetic Engineering
Duration	Content
Module-1 January 2023	Isolation of DNA (Gene of Interest) Fragments to be cloned: ● Literature survey of Related topic ● Preparation of Culture Media for E. Coli Culture ● Isolation of Pure Culture of E. Coli ● Genomic DNA Isolation from host to get desired gene ● Restriction digestion and Southern Blotting to get desired gene
Module-2 February 2023	Insertion of Isolated DNA into a Suitable Vector to Form the Re-combinant DNA: ● Isolation and Selection of a suitable plasmid Vectorsmid Vector ● Restriction Digestion of Cloning vector and Gene of Interestnd Gene of Interest ● Construction of recombinant DNA plasmid
Module-3 March 2023	Introduction of the Recombinant DNA into a Suitable Organism known as Host: ● Preparation of Competent E. coli cells ● Transformation of Recombinant plasmid into host cells ● Selection of transformed and non-transformed cells
Module-4 April 2023	Selection of the Transformed Host Cells and Identification of the Clone Containing the Gene of Interest: ● Selection of transformed and non-transformed cells ● Identification of Recombinant and Non-recombinant cells ● Multiplication/Expression of the Introduced Gene in the Host ● Isolation of the Multiplied Gene Copies/Protein Expressed by the Intro¬duced Gene
Module-5 May 2023	● Review of literature to collect data for report ● Dissertation report writing ● Corrections and editing
Module-6 June 2023	● Preparation of manuscript for publication ● Corrections, editing and submission to Journal

	Topic-IV A Study on Blotting Techniques and Their Applications
Duration	Content
Module-1 January 2023	● Literature survey of Related different blotting Techniques ● Preparation of Culture Media for bacterial culture ● Isolation of Pure Culture of E. coli ● Genomic DNA Isolation from host to get desired gene
Module-2 February 2023	Southern blotting and its application: ● Preparation of samples to perform southern blot technique ● Restriction Digestion of Genomic DNA contain desired gene ● Electrophoresis of digested DNA fragments ● Denaturation and blotting on membrane ● Hybridization with labeled probes and Autoradiography
Module-3 March 2023	Northern blotting and its application: ● RNA isolation (total or poly(A) RNA) ● Probe generation. ● Denaturing agarose gel electrophoresis ● Transfer to solid support and immobilization ● Pre-hybridization and hybridization with probe ● Washing and Detection ● Stripping and re-probing (optional)
Module-4 April 2023	Western blotting and its application: ● Extraction of protein ● Gel electrophoresis: SDS PAGE ● Blotting: electrical or capillary blotting ● Blocking: BSA ● Treatment with primary antibody ● Treatment with secondary antibody (enzyme labelled anti Ab) ● Treatment with specific substrate; if enzyme is alkaline phos-phatase, substrate is p-nitro phenyl phosphate which give color
Module-5 May 2023	● Review of literature to collect data for report ● Dissertation report writing ● Corrections and editing
Module-6 June 2023	● Preparation of manuscript for publication ● Corrections, editing and submission to Journal

	Topic V-Mushroom Cultivation
Duration	Content
Module-1 January 2023	● Literature survey of Related articles ● Preparation of Culture Media and grow fungal culture ● Preparation of mother culture ● Preparation of Spawn culture
Module-2 February 2023	● Collection of different types of substrates used in cultivation ● Treatment of substrates by autoclaving or pasteurization ● Preparation of compost of different substrates
Module-3 March 2023	● Preparation of bags with spawn culture ● Incubation of bags under appropriate environment condition ● Observation till Sprouting of mushrooms from bags ● Harvesting and packing of Mushroom
Module-4 April 2023	Biochemical analysis of sporocarp ● Protein estimation ● Extraction and estimation of carbohydrate ● Determination of lipid content ● Determination of moisture content ● Amino acid detection and separation by TLC ● Antioxidant activity ● Antidiabetic activity
Module-5 May 2023	● Review of literature to collect data for report ● Dissertation report writing ● Corrections and editing
Module-6 June 2023	● Preparation of manuscript for publication ● Corrections, editing and submission to Journal

	Topic VI-Vermicomposting
Duration	Content
Module-1 January 2023	● Literature survey of Related articles ● Preparation of pits or Set up Worm Bin ● Putting the food or waste materials into bins
Module-2 February 2023	● Buying the appropriate strain of Earthworm ● Set up the culture of earthworm in waste ● Monitoring for all the parameters of vermicomposting
Module-3 March 2023	● Harvesting of compost by separating Earthworms ● Re set up of another unit using separated earthworms ● Quality check of compost by appropriate methods
Module-4 April 2023	● Packing or storage of plant material ● Supply of vermicomposting to local market
Module-5 May 2023	● Review of literature to collect data for report ● Dissertation report writing ● Corrections and editing
Module-6 June 2023	● Preparation of manuscript for publication ● Corrections, editing and submission to Journal

	Topic VII-A Study on Hydroponic System
Duration	Content
Module-1 January 2023	● Literature survey of Related articles ● Selection of a place for setup of hydroponic system ● Preparation of media used for hydroponic ● Preparation of plants or plantlets to grow on hydroponic sys-tem
Module-2 February 2023	● Set up of system with required plants ● Monitoring of plant growth and growth conditions ● Application of different small molecules or drugs in liquid me-dia
Module-3 March 2023	● Observation of drug effect by looking at plant growth and phenotype ● Analysis of various chemical and biochemical parameters
Module-4 April 2023	● Harvesting of the grown plant materials ● Packing or storage of plant material
Module-5 May 2023	● Review of literature to collect data for report ● Dissertation report writing ● Corrections and editing
Module-6 June 2023	● Preparation of manuscript for publication ● Corrections, editing and submission to Journal

	Topic VIII- Evaluation of the Immunomodulatory Activity of Plants
Duration	Content
Module-1 January 2023	● Literature survey of Related topic ● Collection of Plant sample ● Preparation of plant extracts using solvent by sox let method
Module-2 February 2023	● Isolation of peripheral Human blood ● Culture of peripheral Human lymphocytes
Module-3 March 2023	● Determination of phytochemical properties of plant extract ● Isolation and analysis of phytochemical from various plant extract
Module-4 April 2023	● Determination of immunomodulatory activity in peripheral blood ● Determination of antioxidant properties
Module-5 May 2023	● Review of literature to collect data for report ● Dissertation report writing ● Corrections and editing
Module-6 June 2023	● Preparation of manuscript for publication ● Corrections, editing and submission to Journal

	Topic IX- Evaluating the Nanomaterial Toxicity on Healthy Human Blood Cells
Duration	Content
Module-1 January 2023	● Literature survey of Related topic ● Identification and preparation of nanomaterial of interest ● Determination of structural characteristics of nanomaterial
Module-2 February 2023	● Isolation of peripheral Human blood ● Culture of peripheral Human lymphocytes ● Invitro analysis of inflammatory and immune response mole-cules
Module-3 March 2023	● Invitro estimation of cellular response after nanomaterial treatment. ● Determination of cytotoxicity of nanomaterial ● Analysis of oxidative damages
Module-4 April 2023	● Analysis of genotoxicity and morphological changes after treating with nanomaterial of cells. ● In-vivo estimation of Nano toxicity using a mouse model
Module-5 May 2023	● Review of literature to collect data for report ● Dissertation report writing ● Corrections and editing
Module-6 June 2023	● Preparation of manuscript for publication ● Corrections, editing and submission to Journal

	Topic X- In-vitro Antimitotic Activity of Whole Plant Extract and its Metabolite Profile
Duration	Content
Module-1 January 2023	● Literature survey of Related topic ● Collection of Plant sample ● Preparation of plant extracts using solvent by soxhlet method
Module-2 February 2023	● Determination of phytochemical properties of plant extract ● Isolation and analysis of phytochemicals from various plant ex-tracts ● Germination of seeds or plants
Module-3 March 2023	● Performing Allium cepa root tip assay ● Preparation and Mounting of slides ● Data collection and statistical analysis
Module-4 April 2023	● Performing germinated seedling root tip assay ● Preparation and Mounting of slides ● Data collection and statistical analysis
Module-5 May 2023	● Review of literature to collect data for report ● Dissertation report writing ● Corrections and editing
Module-6 June 2023	● Preparation of manuscript for publication ● Corrections, editing and submission to Journal

Resource Persons